The "Fish & Chips" project: Microarrays as a tool for the identification of marine organisms in biodiversity and ecosystem research
Kochzius, Marc,
Kappel, Kristina,
Dobitz, Lutz,
Silkenbeumer, Nina,
Nolte, Manfred,
Weber, Hannes,
Hjorleifsdottir, Sigridur,
Marteinsson, Viggo,
Hreggvidsson, Gudmundur,
Planes, Serge,
Tinti, Fausto,
Magoulas, Antonios,
Vazquez, Eva Garcia,
Turan, Cemal,
Medlin, Linda,
Metfies, Katja,
Gescher, Christine,
Cariani, Alessia,
Landi, Monica,
Hervet, Caroline,
Falgueras, Daniel Campo,
Antoniou, Aglaia,
Bertasi, Fa,
Srujana, Chitipothu and
Blohm, Dietmar
;
Contact
kochzius [ at ] uni-bremen.de
Abstract
DNA microarrays are currently in use almost exclusively as research tools for gene expression analysis and their application for the identification of organisms is still in its infancy, only documented by a few studies on mammals, bacteria, and viruses. The "Fish & Chips" project aims to demonstrate that DNA chips can be a new innovative tool for the identification of marine animals and phytoplankton. To achieve this goal, fishes, invertebrates, and phytoplankton were sampled in European seas and taxonomically classified. Fragments of their mitochondrial 16S, cyt b, and COI genes and from the nuclear 18S genes were sequenced and served as molecular markers to enable probe design for the microarrays. An on-line data base containing the sequences and all relevant information of the samples has been implemented. A first prototype of a "Fish Chip",is based on more than 400 sequences from the 16S rRNA gene belonging to 46 species. A second microarray prototype serves to identify flatfishes from the North Sea based on COI-and 16S sequences from 70 individuals of 17 fish species. A "Phytoplankton Chip" is now available with probes for all microalgal classes and many toxic species, as well as a "Invertebrate Chip". The results show that this approach is feasible. © 2007 IEEE.
Item Type
Conference
(Paper)
Authors
Kochzius, Marc,
Kappel, Kristina,
Dobitz, Lutz,
Silkenbeumer, Nina,
Nolte, Manfred,
Weber, Hannes,
Hjorleifsdottir, Sigridur,
Marteinsson, Viggo,
Hreggvidsson, Gudmundur,
Planes, Serge,
Tinti, Fausto,
Magoulas, Antonios,
Vazquez, Eva Garcia,
Turan, Cemal,
Medlin, Linda,
Metfies, Katja,
Gescher, Christine,
Cariani, Alessia,
Landi, Monica,
Hervet, Caroline,
Falgueras, Daniel Campo,
Antoniou, Aglaia,
Bertasi, Fa,
Srujana, Chitipothu and
Blohm, Dietmar
;
Divisions
Programs
Publication Status
Published
Event Details
Oceans 2007 : Aberdeen, ScotlandJune 2007 / [IEEE]. Piscataway, NJ : IEEE Operations Center.
Eprint ID
17967
DOI
https://www.doi.org/10.1109/oceanse.2007.4302414
Cite as
Kochzius, M.
,
Kappel, K.
,
Dobitz, L.
,
Silkenbeumer, N.
,
Nolte, M.
,
Weber, H.
,
Hjorleifsdottir, S.
,
Marteinsson, V.
,
Hreggvidsson, G.
,
Planes, S.
,
Tinti, F.
,
Magoulas, A.
,
Vazquez, E. G.
,
Turan, C.
,
Medlin, L.
,
Metfies, K.
,
Gescher, C.
,
Cariani, A.
,
Landi, M.
,
Hervet, C.
,
Falgueras, D. C.
,
Antoniou, A.
,
Bertasi, F.
,
Srujana, C.
and
Blohm, D.
(2007):
The "Fish & Chips" project: Microarrays as a tool for the identification of marine organisms in biodiversity and ecosystem research
,
Oceans 2007 : Aberdeen, ScotlandJune 2007 / [IEEE]. Piscataway, NJ : IEEE Operations Center
.
doi: https://www.doi.org/10.1109/oceanse.2007.4302414
Share
Citation
Research Platforms
Campaigns
N/A
Actions
Edit Item