Environmental isotope techniques, hydrogeochemical analysis and hydraulic data are employed to identify the main recharge areas of the Mt. Vulture hydrogeological basin, one of the most important aquifers of southern Italy. The groundwaters are derived from seepage of rainwater, flowing from the highest to the lowest elevations through the shallow volcanic weathered host-rock fracture zones. Samples of shallow and deep groundwater were collected at 48 locations with elevations ranging from 352 to 1,100m above sea level (a. s. l.), for stable isotope (δ18O, δD) and major ion analyses. A complete dataset of available hydraulic information has been integrated with measurements carried out in the present study. Inferred recharge elevations, estimated on the basis of the local vertical isotopic gradient of δ18O, range between 550 and 1,200m a. s. l. The isotope pattern of the Quaternary aquifer reflects the spatial separation of different recharge sources. Knowledge of the local hydrogeological setting was the starting point for a detailed hydrogeochemical and isotopic study to define the recharge and discharge patterns identifying the groundwater flow pathways of the Mt. Vulture basin. The integration of all the data allowed for the tracing of the groundwater flows of the Mt. Vulture basin. © 2010 Springer-Verlag.