<jats:p> Temperature reconstructions indicate that the Pliocene was approximately 3 <jats:sup>°</jats:sup> C warmer globally than today, and several recent reconstructions of Pliocene atmospheric CO <jats:sub>2</jats:sub> indicate that it was above pre-industrial levels and similar to those likely to be seen this century. However, many of these reconstructions have been of relatively low temporal resolution, meaning that these records may have failed to capture variations associated with the 41 kyr glacial–interglacial cycles thought to have operated in the Pliocene. Here we present a new, high temporal resolution alkenone carbon isotope-based record of <jats:italic>p</jats:italic> CO <jats:sub>2</jats:sub> spanning 3.3–2.8 Ma from Ocean Drilling Program Site 999. Our record is of high enough resolution (approx. 19 kyr) to resolve glacial–interglacial changes beyond the intrinsic uncertainty of the proxy method. The record suggests that Pliocene CO <jats:sub>2</jats:sub> levels were relatively stable, exhibiting variation less than 55 ppm. We perform sensitivity studies to investigate the possible effect of changing sea surface temperature (SST), which highlights the importance of accurate and precise SST reconstructions for alkenone palaeobarometry, but demonstrate that these uncertainties do not affect our conclusions of relatively stable <jats:italic>p</jats:italic> CO <jats:sub>2</jats:sub> levels during this interval. </jats:p>