<jats:p>During diversity studies of the glacier forefields of the Larsemann Hills, East Antarctica, a novel psychrotolerant, non-motile Gram-negative, shiny yellow, rod-shaped, aerobic bacterium, designated strain PB4<jats:sup>T</jats:sup> was isolated from a soil sample. Strain PB4<jats:sup>T</jats:sup> produces indole from tryptophan and hydrolyses casein. It grows between 0 and 25 °C with an optimum growth temperature of 20 °C. A wide range of substrates are used as sole carbon sources and acid is produced from numerous carbohydrates. The major menaquinone is MK-6. Identified polar lipids are ethanolamines and ornithine lipids. Major fatty acids (&gt;10 %) are iso-C<jats:sub>15 : 0</jats:sub> (13.0 %) and iso-2OH-C<jats:sub>15 : 0</jats:sub> (51.2 %). G+C content is 33.7 mol%. The polyamine pattern is composed of sym-homospermidine (25.1 µmol g<jats:sup>−1</jats:sup> dry weight), minor amounts of cadaverine (0.2 µmol g<jats:sup>−1</jats:sup> dry weight) and spermidine (0.4 µmol g<jats:sup>−1</jats:sup> dry weight) and traces of putrescine and spermine (&lt;0.1 µmol g<jats:sup>−1</jats:sup> dry weight). Strain PB4<jats:sup>T</jats:sup> had highest 16S rRNA gene similarities with the type strains of <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="species" xlink:type="simple"> <jats:ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1601/nm.16885" xlink:type="simple"> <jats:italic>Chryseobacterium humi</jats:italic> </jats:ext-link> </jats:named-content> (97.0 %) and <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="species" xlink:type="simple"> <jats:ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1601/nm.14657" xlink:type="simple"> <jats:italic>Chryseobacterium marinum</jats:italic> </jats:ext-link> </jats:named-content> (96.5 %). Considering phenotypic and genotypic characterization, strain PB4<jats:sup>T</jats:sup> represents a novel species in the genus <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus" xlink:type="simple"> <jats:ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1601/nm.8147" xlink:type="simple"> <jats:italic>Chryseobacterium</jats:italic> </jats:ext-link> </jats:named-content> (family <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="family" xlink:type="simple"> <jats:ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1601/nm.8070" xlink:type="simple"> <jats:italic>Flavobacteriaceae</jats:italic> </jats:ext-link> </jats:named-content>), for which the name <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus" xlink:type="simple"> <jats:ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1601/nm.8147" xlink:type="simple"> <jats:italic>Chryseobacterium</jats:italic> </jats:ext-link> </jats:named-content> <jats:italic>frigidisoli</jats:italic> sp. nov. is proposed. The type strain is PB4<jats:sup>T</jats:sup> ( = DSM 26000<jats:sup>T</jats:sup> = LMG 27025<jats:sup>T</jats:sup>).</jats:p>