Molecular discrimination of taxa within the dinoflagellate genus Azadinium, the source of azaspiracid toxins

Toebe, Kerstin, Joshi, Aboli R, Messtorff, Philip, Tillmann, Urban, Cembella, Allan and John, Uwe ;

Contact

Kerstin.Toebe [ at ] awi.de

Abstract

Molecular probes were developed for the dinoflagellate genus Azadinium to discriminate among three taxa difficult to differentiate by light microscopy. This genus contains azaspiracid toxin-producing Azadinium spinosum, but also non-toxigenic species. Quantitative polymerase chain reaction (qPCR) and fluorescence in situ hybridization (FISH) assays were applied to cultured isolates and Azadinium-spiked field plankton. Molecular methods were highly specific and sensitive in the unambiguous detection of Azadinium, and thus are valuable for routine plankton, biogeographic and phylogenetic investigations. © 2012 The Author 2012. Published by Oxford University Press. All rights reserved.

Item Type

Article

Authors

Toebe, Kerstin, Joshi, Aboli R, Messtorff, Philip, Tillmann, Urban, Cembella, Allan and John, Uwe ;

Divisions

AWI Organizations > Biosciences > Ecological Chemistry

Programs

Helmholtz Research Programs > PACES I (2009-2013) > TOPIC 2: Coastal Change > WP 2.1: Food Webs and Diversity under Global and Regional Change

Publication Status

Published

Eprint ID

31960

DOI https://www.doi.org/10.1093/plankt/fbs077

Cite as

Toebe, K. , Joshi, A. R. , Messtorff, P. , Tillmann, U. , Cembella, A. and John, U. (2013): Molecular discrimination of taxa within the dinoflagellate genus Azadinium, the source of azaspiracid toxins , Journal of Plankton Research, 35 (1), pp. 225-230 . doi: https://www.doi.org/10.1093/plankt/fbs077

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