Identification of the marine diatomPseudo-nitzschia multiseries(Bacillariophyceae) as a source of the toxin domoic acid in Algoa Bay, South Africa


Contact
Bernd.Krock [ at ] awi.de

Abstract

A unialgal culture of a Pseudo-nitzschia species dominant in the plankton of Algoa Bay in the spring of 2012 was established by isolation of clonal chains of cells. Identification of the species as Pseudo-nitzschia multiseries was based on frustule morphometrics provided by light and scanning electron microscopy, and confirmed by phylogenetic analysis of the LSU rDNA gene. Cultures were shown to produce domoic acid (DA) as measured by ELISA and LC/MS-MS methods, and levels of cellular DA were ∼0.1 pg cell<sup>–1</sup>. Although it is recognised as a cosmopolitan species, these observations provide the first account of this toxic diatom in the coastal waters of South Africa.



Item Type
Article
Authors
Divisions
Primary Division
Programs
Primary Topic
Publication Status
Published
Eprint ID
37094
DOI https://www.doi.org/10.2989/1814232x.2014.983971

Cite as
Pitcher, G. , Cembella, A. , Krock, B. , Macey, B. , Mansfield, L. and Probyn, T. (2014): Identification of the marine diatomPseudo-nitzschia multiseries(Bacillariophyceae) as a source of the toxin domoic acid in Algoa Bay, South Africa , African Journal of Marine Science, 36 (4), pp. 523-528 . doi: https://www.doi.org/10.2989/1814232x.2014.983971


Download
[thumbnail of Pitcher_2014.pdf]
Preview
PDF
Pitcher_2014.pdf

Download (1MB) | Preview
Cite this document as:

Share


Citation

Geographical region

Research Platforms
N/A

Campaigns
N/A


Actions
Edit Item Edit Item