Non-destructive DNA extraction for small pelagic copepods to perform integrative taxonomy

Cornils, Astrid

Contact

Astrid.Cornils [ at ] awi.de

Abstract

Molecular analyses of small-sized copepods (≤ 1 mm) generally involve the complete destruction of the specimens. Consequently, incongruences between the molecular and morphological results cannot be investigated since no specimen vouchers remain. The present study provides a modified column-based DNA extraction method to retain the exoskeleton of the specimen and thus, to enable molecular and morphological analysis of the same specimens. The method has been tested on ethanol preserved specimens of nine pelagic copepod genera.

Item Type

Article

Authors

Cornils, Astrid

Divisions

AWI Organizations > Biosciences > Bentho-Pelagic Processes

Primary Division

Organizations > AWI Organizations > Biosciences > Bentho-Pelagic Processes

Programs

Basic Research > Helmholtz Independent Research

Primary Topic

Helmholtz Programs > Basic Research > Helmholtz Independent Research

Peer revision

ISI/Scopus peer-reviewed

Publication Status

Published

Eprint ID

37259

DOI https://www.doi.org/10.1093/plankt/fbu105

Cite as

Cornils, A. (2015): Non-destructive DNA extraction for small pelagic copepods to perform integrative taxonomy , Journal of Plankton Research, 37 (1), pp. 6-10 . doi: https://www.doi.org/10.1093/plankt/fbu105

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hdl:10013/epic.44965.d001

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