Anti-Fouling Effects of Saponin-Containing Crude Extracts from Tropical Indo-Pacific Sea Cucumbers


Contact
berenike.bick [ at ] hifmb.de

Abstract

<jats:p>Sea cucumbers are bottom dwelling invertebrates, which are mostly found on subtropical and tropical sea grass beds, sandy reef flats, or reef slopes. Although constantly exposed to fouling communities in these habitats, many species are surprisingly free of invertebrate epibionts and microfouling algae such as diatoms. In our study, we investigated the anti-fouling (AF) activities of different crude extracts of tropical Indo-Pacific sea cucumber species against the fouling diatom Cylindrotheca closterium. Nine sea cucumber species from three genera (i.e., Holothuria, Bohadschia, Actinopyga) were selected and extracted to assess their AF activities. To verify whether the sea cucumber characteristic triterpene glycosides were responsible for the observed potent AF activities, we tested purified fractions enriched in saponins isolated from Bohadschia argus, representing one of the most active anti-fouling extracts. Saponins were quantified by vanillin-sulfuric acid colorimetric assays and identified by LC-MS and LC-MS/MS analyses. We were able to demonstrate that AF activities in sea cucumber extracts were species-specific, and growth inhibition as well as attachment of the diatom to surfaces is dependent on the saponin concentration (i.e., Actinopyga contained the highest quantities), as well as on the molecular composition and structure of the present saponins (i.e., Bivittoside D derivative was the most bioactive compound). In conclusion, the here performed AF assay represents a promising and fast method for selecting the most promising bioactive organism as well as for identifying novel compounds with potent AF activities for the discovery of potentially novel pharmacologically active natural products.</jats:p>



Item Type
Article
Authors
Divisions
Primary Division
Programs
Primary Topic
Publication Status
Published
Eprint ID
52314
DOI https://www.doi.org/10.3390/md18040181

Cite as
Kamyab, E. , Goebeler, N. , Kellermann, M. Y. , Rohde, S. , Reverter, M. , Striebel, M. and Schupp, P. J. (2020): Anti-Fouling Effects of Saponin-Containing Crude Extracts from Tropical Indo-Pacific Sea Cucumbers , Marine Drugs, 18 (4), p. 181 . doi: https://www.doi.org/10.3390/md18040181


Download
[thumbnail of Kamyab_et_al_2020.pdf]
Preview
PDF
Kamyab_et_al_2020.pdf

Download (3MB) | Preview

Share


Citation

Geographical region
N/A

Research Platforms
N/A

Campaigns
N/A


Actions
Edit Item Edit Item